Depending on demand, the budget airline will look to roll out the device across its entire domestic and international network later in the year.
The iPads will provide in-flight entertainment with movies, TV programs, eBooks, music videos, games and CDs and will cost passengers $10 to rent on board.
Jetstar CEO Bruce Buchanan said it anticipates huge demand for the mobile device.
He added: "We are really excited to commence trialing the iPads, as we're always looking for ways to enhance our customer's Jetstar experience. Given the demand for the iPad so far, I anticipate it will have strong appeal amongst our passengers.”